Take the width of the dirt road as 1 unit and measure everything in-regards to that.
If you have a photoshop you can use measure tool. First of all you need to create scale, like "1 building = 1 module" so when measuring for example you'll get values that something on the map is "3,45 modules" long.
Thanks guys, this really helps 
I would scale the map until the straight angles (walls and such) are all 45 degrees. Then I would rotate the map 45 degrees to get a map to use as base. Made a quick illustration of this. The good thing is that you would be able to bring this into a 3dpackage and extrude shapes directly from the map layout.
Great tips, guys. I would have never thought such techniques.
Isometric is strange compared to (what I am used to, anyway) regular projections. You may even be able to create a vanishing point to create a single "unit", & then flatten it, so it stays the same scale in spite of depth.
there are no vanishing points in isometrics, and there is no real depth or change of scale across an isometric projection.
the whole point of isometrics is, originally, measurability, so that once you know one measurement along either the x, y, or z axis, you can reliably measure anything else that lies on those axes (this has already been said).
i know that's not as helpful as some of the other posts, but i thought i'd clear that up.
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